A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis.
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منابع مشابه
A gene encoding proline dehydrogenase is not only induced by proline and hypoosmolarity, but is also developmentally regulated in the reproductive organs of Arabidopsis.
The cDNA clone ERD5 (early responsive to dehydration), isolated from 1-h-dehydrated Arabidopsis, encodes a precursor of proline (Pro) dehydrogenase (ProDH), which is a mitochondrial enzyme involved in the first step of the conversion of Pro to glutamic acid. The transcript of the erd5 (ProDH) gene was undetectable when plants were dehydrated, but large amounts of transcript accumulated when pla...
متن کاملProline Metabolism in Escherichia Coli. Ii. Regulation of Total Growth of a Proline Auxotroph by a Proline-oxidizing System.
Frank, Leonard (The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Md.). Proline metabolism in Escherichia coli. II. Regulation of total growth of a proline auxotroph by a proline-oxidizing system. J. Bacteriol. 86:781-784. 1963.-With a simple kinetic model, it was shown that a proline-requiring mutant of Escherichia coli was subject to growth limitation on proline by ...
متن کاملAltered levels of proline dehydrogenase cause hypersensitivity to proline and its analogs in Arabidopsis.
Pro dehydrogenase (PDH) catalyzes the first and rate-limiting step in the Pro catabolic pathway. In Arabidopsis, this enzyme is encoded by At-PDH. To investigate the role of Pro catabolism in plants, we generated transgenic Arabidopsis plants with altered levels of PDH by sense (PDH-S plants) and antisense (PDH-AS plants) strategies. Free Pro levels were reduced by up to 50% in PDH-S plants und...
متن کاملProduction of Recombinant Proline Dehydrogenase Enzyme from Pseudomonas fluorescens pf-5 in E. coli System
Proline dehydrogenase (ProDH; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. The goal of this research was gene cloning and characterization of ProDH enzyme from Pseudomonas fluorescens pf-5 strain. The gene encoding ProDH was isolated by means of PCR amplification and cloned in an IPTG inducible T...
متن کاملProline dehydrogenase: a key enzyme in controlling cellular homeostasis.
Proline dehydrogenase (ProDH), also called proline oxidase (POX), is a universal enzyme in living organisms. It catalyzes the oxidation of L-proline to delta1-pyrroline-5-carboxylate leading to the release of electrons, which can be transferred to either electron transfer systems or to molecular oxygen. ProDH is not only essential for proline catabolism but also plays key roles in providing ene...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1996
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.8.8.1323